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p erm  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p erm
    P Erm, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+erm/pmc13010088-105-27-29
    Average 86 stars, based on 1 article reviews
    p erm - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: NADPH Oxidase 5 (NOX5) Overexpression Promotes Endothelial Dysfunction via Cell Apoptosis, Migration, and Metabolic Alterations in Human Brain Microvascular Endothelial Cells (hCMEC/D3)
    Article Snippet: For protein immunoblot detection overnight incubation at 4 °C was performed with the following antibodies: 1:5000 β-actin (A5441, Sigma Aldrich ® ) 5% milk in PBS, 1:500 NOX5 (191010, Abcam ® , Waltham, MA, USA) 5% milk in 0.05% Tween-PBS, 1:500 ERM (3142S, Cell Signaling TM , Danvers, MA, USA) 1% BSA in 0.05% Tween-PBS and 1:500 P-ERM (Phospho-Ezrin(Thr567)/Radixin(Thr564)/Moesin(Thr558) (3141S, Cell Signaling TM ) 1% BSA in 0.05% Tween-PBS.

    Article Title: CU104, a novel barrier function enhancer, improves colitis via modulation of barrier function and immune cell recruitment
    Article Snippet: Cells or deparaffinized 4-μm tissue sections were fixed with 10% formalin (pH 7.4), permeabilized with 0.5% Triton X-100 in PBS (PBS-T), and blocked with serum-free protein block (Dako #X0909, Carpinteria, CA, USA).

    Staining:

    Article Title: RSPO2 Coordinates with GDF9:BMP15 Heterodimers to Promote Granulosa Cell and Oocyte Development in Mice.
    Article Snippet: DNA was counterstained with Hoechst 33342 (2 μg mL−1) (Cat#14533, Sigma–Aldrich) for 10 min. .. The primary antibodies used for ovarian IF staining included: KI-67 (Cat# ab16667, 1:200, Abcam, Cambridge, Cambridgeshire, UK); ZP2 (Cat# sc-32752, 1:500, Santa Cruz Biotechnology, Dallas, Texas, USA); γ-H2A.X (Cat# BS4760, 1:1000, Bioworld Technology, Dublin, Ohio, USA); DDX4 (Cat# ab13840, 1:500, Abcam); FOXL2 (Cat# NB100-1277, 1:300, Novus Biologicals, Littleton, Colorado, USA); RDX (Cat# F1012, 1:200, Selleck Chemicals, Houston, Texas, USA); p-ERM (Cat# 3726, 1:200, Cell Signaling Technology, Danvers, Massachusetts, USA); Active-CTNNB1 (Cat# 05–665, 1:500, SigmaAldrich); AMH (Cat# 14461-1-AP, 1:200, Proteintech, Wuhan, Hubei, China); and Phospho-SMAD2 pSer465/pSer467 (Cat# 40–0800, 1:200, Thermo Fisher Scientific). ..

    Article Title: RSPO2 Coordinates with GDF9:BMP15 Heterodimers to Promote Granulosa Cell and Oocyte Development in Mice
    Article Snippet: DNA was counterstained with Hoechst 33342 (2 μg mL −1 ) (Cat#14533, Sigma–Aldrich) for 10 min. .. The primary antibodies used for ovarian IF staining included: KI‐67 (Cat# ab16667, 1:200, Abcam, Cambridge, Cambridgeshire, UK); ZP2 (Cat# sc‐32752, 1:500, Santa Cruz Biotechnology, Dallas, Texas, USA); γ‐H2A.X (Cat# BS4760, 1:1000, Bioworld Technology, Dublin, Ohio, USA); DDX4 (Cat# ab13840, 1:500, Abcam); FOXL2 (Cat# NB100‐1277, 1:300, Novus Biologicals, Littleton, Colorado, USA); RDX (Cat# F1012, 1:200, Selleck Chemicals, Houston, Texas, USA); p‐ERM (Cat# 3726, 1:200, Cell Signaling Technology, Danvers, Massachusetts, USA); Active‐CTNNB1 (Cat# 05–665, 1:500, Sigma‐Aldrich); AMH (Cat# 14461‐1‐AP, 1:200, Proteintech, Wuhan, Hubei, China); and Phospho‐SMAD2 pSer465/pSer467 (Cat# 40–0800, 1:200, Thermo Fisher Scientific). ..

    Article Title: Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway
    Article Snippet: .. For immunofluorescence staining, the following antibodies were used: anti-MAP1LC3B (Cell Signaling Technology, 2775 and Santa Cruz Biotechnology, sc-271625), anti-BNIP3L (Cell Signaling Technology, 12396), anti-CA9 (Invitrogen, MA5-16318), p-ERM (3726S), and anti-POU5F1/OCT4 (Invitrogen, MA5-31458 and Abcam, ab18976). ..

    Western Blot:

    Article Title: TNIK: A redox sensor in endothelial cell permeability.
    Article Snippet: .. Anti–p- ERM/anti–p- Dmoesin (Cell Signaling Technology, 3141), anti- GST (ChromoTek, 6G9), anti–p- Thr (Cell Signaling Technology, 9381), anti- GFP for Western blotting (ChromoTek, 3H9), antiGFP for immunofluorescence detection of YFP-TNIK (Roche, 11814460001), anti- TNIK (Santa Cruz Biotechnology, sc- 136103), anti- Actin (Sigma- Aldrich, A2228), anti–p- MLC2 Ser19 (Cell Signaling Technology, 3671), anti- Ezrin (Cell Signaling Technology, 3145), anti- moesin (Cell Signaling Technology, 3150), anti- ERM (Cell Signaling Technology, 3142), anti- vascular endothelial (VE)cadherin for immunofluorescence (R&D Systems, goat polyclonal, AF938), anti–VE- cadherin for Western blotting (Santa Cruz Biotechnology, sc- 6458), anti–glyceraldehyde- 3- phosphate dehydrogenase (GAPDH) (Sigma- Aldrich, G9545), and anti–Histone H3 (Cell Signaling Technology, 9715). .. Cell culture of derived and primary cell lines HUVECs from pooled donors (Lonza, Verviers Belgium or PromoCell, Heidelberg Germany) were cultured in Endothelial Growth Medium (EGM)- 2 medium (Lonza) in a humidified incubator at 37°C in 5% CO2 up to four passages.

    Article Title: TNIK: A redox sensor in endothelial cell permeability
    Article Snippet: .. Anti–p-ERM/anti–p-Dmoesin (Cell Signaling Technology, 3141), anti-GST (ChromoTek, 6G9), anti–p-Thr (Cell Signaling Technology, 9381), anti-GFP for Western blotting (ChromoTek, 3H9), anti-GFP for immunofluorescence detection of YFP-TNIK (Roche, 11814460001), anti-TNIK (Santa Cruz Biotechnology, sc-136103), anti-Actin (Sigma-Aldrich, A2228), anti–p-MLC2 Ser 19 (Cell Signaling Technology, 3671), anti-Ezrin (Cell Signaling Technology, 3145), anti-moesin (Cell Signaling Technology, 3150), anti-ERM (Cell Signaling Technology, 3142), anti-vascular endothelial (VE)-cadherin for immunofluorescence (R&D Systems, goat polyclonal, AF938), anti–VE-cadherin for Western blotting (Santa Cruz Biotechnology, sc-6458), anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich, G9545), and anti–Histone H3 (Cell Signaling Technology, 9715). .. HUVECs from pooled donors (Lonza, Verviers Belgium or PromoCell, Heidelberg Germany) were cultured in Endothelial Growth Medium (EGM)-2 medium (Lonza) in a humidified incubator at 37°C in 5% CO 2 up to four passages.

    Immunofluorescence:

    Article Title: TNIK: A redox sensor in endothelial cell permeability.
    Article Snippet: .. Anti–p- ERM/anti–p- Dmoesin (Cell Signaling Technology, 3141), anti- GST (ChromoTek, 6G9), anti–p- Thr (Cell Signaling Technology, 9381), anti- GFP for Western blotting (ChromoTek, 3H9), antiGFP for immunofluorescence detection of YFP-TNIK (Roche, 11814460001), anti- TNIK (Santa Cruz Biotechnology, sc- 136103), anti- Actin (Sigma- Aldrich, A2228), anti–p- MLC2 Ser19 (Cell Signaling Technology, 3671), anti- Ezrin (Cell Signaling Technology, 3145), anti- moesin (Cell Signaling Technology, 3150), anti- ERM (Cell Signaling Technology, 3142), anti- vascular endothelial (VE)cadherin for immunofluorescence (R&D Systems, goat polyclonal, AF938), anti–VE- cadherin for Western blotting (Santa Cruz Biotechnology, sc- 6458), anti–glyceraldehyde- 3- phosphate dehydrogenase (GAPDH) (Sigma- Aldrich, G9545), and anti–Histone H3 (Cell Signaling Technology, 9715). .. Cell culture of derived and primary cell lines HUVECs from pooled donors (Lonza, Verviers Belgium or PromoCell, Heidelberg Germany) were cultured in Endothelial Growth Medium (EGM)- 2 medium (Lonza) in a humidified incubator at 37°C in 5% CO2 up to four passages.

    Article Title: Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway
    Article Snippet: .. For immunofluorescence staining, the following antibodies were used: anti-MAP1LC3B (Cell Signaling Technology, 2775 and Santa Cruz Biotechnology, sc-271625), anti-BNIP3L (Cell Signaling Technology, 12396), anti-CA9 (Invitrogen, MA5-16318), p-ERM (3726S), and anti-POU5F1/OCT4 (Invitrogen, MA5-31458 and Abcam, ab18976). ..

    Article Title: TNIK: A redox sensor in endothelial cell permeability
    Article Snippet: .. Anti–p-ERM/anti–p-Dmoesin (Cell Signaling Technology, 3141), anti-GST (ChromoTek, 6G9), anti–p-Thr (Cell Signaling Technology, 9381), anti-GFP for Western blotting (ChromoTek, 3H9), anti-GFP for immunofluorescence detection of YFP-TNIK (Roche, 11814460001), anti-TNIK (Santa Cruz Biotechnology, sc-136103), anti-Actin (Sigma-Aldrich, A2228), anti–p-MLC2 Ser 19 (Cell Signaling Technology, 3671), anti-Ezrin (Cell Signaling Technology, 3145), anti-moesin (Cell Signaling Technology, 3150), anti-ERM (Cell Signaling Technology, 3142), anti-vascular endothelial (VE)-cadherin for immunofluorescence (R&D Systems, goat polyclonal, AF938), anti–VE-cadherin for Western blotting (Santa Cruz Biotechnology, sc-6458), anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich, G9545), and anti–Histone H3 (Cell Signaling Technology, 9715). .. HUVECs from pooled donors (Lonza, Verviers Belgium or PromoCell, Heidelberg Germany) were cultured in Endothelial Growth Medium (EGM)-2 medium (Lonza) in a humidified incubator at 37°C in 5% CO 2 up to four passages.

    Cell-Signaling:

    Article Title: Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway
    Article Snippet: .. For immunofluorescence staining, the following antibodies were used: anti-MAP1LC3B (Cell Signaling Technology, 2775 and Santa Cruz Biotechnology, sc-271625), anti-BNIP3L (Cell Signaling Technology, 12396), anti-CA9 (Invitrogen, MA5-16318), p-ERM (3726S), and anti-POU5F1/OCT4 (Invitrogen, MA5-31458 and Abcam, ab18976). ..



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